![]() The PFO dimer is also an elongated particle whose shape and volume are consistent with a staggered antiparallel dimer. In solution, PLY is elongated, consistent with the shape predicted by its high-resolution homology model. Ab initio dummy atom and dummy residue models for PFO and PLY were restored from the distance distribution function derived from experimental small-angle x-ray scattering curves. AUC data demonstrate that both proteins in solution are mostly monodisperse but PLY is a monomer whereas PFO is mostly dimeric. We report a comparative low-resolution structural study of PLY and PFO using AUC and small-angle x-ray scattering (SAXS). Adam and Hannah brothers, Byron (Beverly) Zingg of McClusky, ND. PFO, a virulence factor of the organism Clostridium perfringens, has almost the same molecular mass as PLY and shares 48% sequence identity and 60% sequence similarity with PLY. Please join us in Loving, Sharing and Memorializing Sharyll B. Current understanding of PLY molecular mechanism is guided by a model built on the basis of its homology with perfringolysin O (PFO) for which there is an atomic structure. PLY remained mostly monomeric in solution intermediate PLY multimers were detected in small quantities. Hydrodynamic bead modeling was used to interpret the data obtained. PLY solution species from monomer via multimeric intermediates to ring-shaped oligomers were studied with time-dependent sedimentation velocity in the analytical ultracentrifuge (AUC). Sharyll was preceded in death by her parents, a sister-in-law, Suzie Zingg and several nieces, nephews and a great niece.Pneumolysin (PLY), an important protein virulence factor of the human bacterial pathogen Streptococcus pneumoniae, could be a candidate for inclusion in a new anti-streptococcal vaccine. She is survived by her husband Clinton, Washburn daughter, Barb (Gust) Stute of Mercer, ND sons, Rob (Bonnie) Tweeten of Hensler, ND, Chad (Bridgett) Tweeten of Washburn 8 grandchildren, Cayce (Nancy), Shaun, Zac, Clay, Luke, Ryan, Logan, AvaBelle and 2 great grandchildren, Adam and Hannah brothers, Byron (Beverly) Zingg of McClusky, ND, Lamont “Monte” Zingg of Turtle Lake sisters, Connie (Arlin) Kemmit of Turtle Lake, Judy (Gary) Presser of Mercer, ND several nieces and nephews special friends, Debbie (Loren) Loose of Underwood, ND and Karen (Richard) Hanson of Turtle Lake. She enjoyed sewing, baking, attending horse shows with Clinton and most of all spending time with family and friends, especially her grandchildren and great grandchildren. She served on the Ingersoll School and Cemetery Board for many years. She was also a member of the Turtle Lake Study Club and the Pioneer Daughters. Sharyll was a member of the Washburn Baptist Church where she served in many capacities. She retired from the nursing home in 2007. She worked as a housekeeper at the Praireview Nursing Home for 20 years. Following their marriage they lived 12 miles N.E. ![]() Sharyll married Clinton Tweeten on Septemat St. ![]() She was raised and educated in Turtle Lake, ND where she graduated from high school in 1959. ![]() Sharyll was born Jin Underwood, ND the daughter of Gordon and Wilhelmena (Johnson) Zingg. Interment will be held in the Riverview Cemetery, Washburn, ND A prayer service will be held at 7:00 PM Tuesday at the church. Visitation will be from 5:00 to 7:00 PM Tuesday at the church and will continue one hour before services on Wednesday at the church. Tweeten, 72, Washburn, ND, died Apat her residence.įuneral Services will be held 10:30 AM Wednesday Apat the Washburn Baptist Church with Pastor Rick Torkelson officiating.
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